X-ray diffraction
1.65Å resolution

Lysozyme contamination facilitates crystallization of a hetero-trimericCortactin:Arg:Lysozyme complex

Primary publication:
Lysozyme contamination facilitates crystallization of a heterotrimeric cortactin-Arg-lysozyme complex.
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 68 154-8 (2012)
PMID: 22297987

Function and Biology Details

Reactions catalysed:
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate

Structure analysis Details

Assembly composition:
Non-polymer only trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
Expression system: Not provided
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme
Src substrate cortactin Chain: B
Molecule details ›
Chain: B
Length: 65 amino acids
Theoretical weight: 7.2 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
  • Canonical: Q60598 (Residues: 487-546; Coverage: 11%)
Gene names: Cttn, Ems1
Sequence domains: Variant SH3 domain
Structure domains: SH3 Domains
Tyrosine-protein kinase ABL2 Chain: C
Molecule details ›
Chain: C
Length: 17 amino acids
Theoretical weight: 1.87 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: P42684 (Residues: 563-579; Coverage: 1%)
Gene names: ABL2, ABLL, ARG

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P212121
Unit cell:
a: 38.658Å b: 57.838Å c: 95.513Å
α: 90° β: 90° γ: 90°
R R work R free
0.186 0.184 0.225
Expression systems:
  • Not provided
  • Escherichia coli