3ulq

X-ray diffraction
2.3Å resolution

Crystal Structure of the Anti-Activator RapF Complexed with the Response Regulator ComA DNA Binding Domain

Released:
Source organism: Bacillus subtilis

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Response regulator aspartate phosphatase F Chain: A
Molecule details ›
Chain: A
Length: 383 amino acids
Theoretical weight: 45.78 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P71002 (Residues: 2-381; Coverage: 100%)
Gene names: BSU37460, rapF, ywhJ
Sequence domains:
Structure domains: Tetratricopeptide repeat domain
Transcriptional regulatory protein ComA Chain: B
Molecule details ›
Chain: B
Length: 90 amino acids
Theoretical weight: 9.97 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P14204 (Residues: 146-214; Coverage: 32%)
Gene names: BSU31680, comA, comA1, comAA
Sequence domains: Bacterial regulatory proteins, luxR family
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21212
Unit cell:
a: 115.304Å b: 81.437Å c: 78.346Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.224
Expression system: Escherichia coli