3uie

X-ray diffraction
1.79Å resolution

Crystal structure of adenosine 5'-phosphosulfate kinase from Arabidopsis Thaliana in Complex with AMPPNP and APS

Released:
Source organism: Arabidopsis thaliana
Primary publication:
Structural basis and evolution of redox regulation in plant adenosine-5'-phosphosulfate kinase.
Proc. Natl. Acad. Sci. U.S.A. 109 309-14 (2012)
PMID: 22184237

Function and Biology Details

Reaction catalysed:
ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylyl-sulfate kinase 1, chloroplastic Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 200 amino acids
Theoretical weight: 22.1 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q43295 (Residues: 77-276; Coverage: 73%)
Gene names: AKN1, APK1, At2g14750, F26C24.11, T6B13.1
Sequence domains: Adenylylsulphate kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 121.137Å b: 95.313Å c: 73.327Å
α: 90° β: 114.08° γ: 90°
R-values:
R R work R free
0.174 0.173 0.201
Expression system: Escherichia coli