PDBe 3ugp

X-ray diffraction
2.7Å resolution

Crystal structure of RNA-polymerase sigma subunit domain 2 complexed with -10 promoter element ssDNA oligo (TATAAT)

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
homo tetramer
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
RNA polymerase sigma factor SigA Chain: A
Molecule details ›
Chain: A
Length: 245 amino acids
Theoretical weight: 28.04 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9EZJ8 (Residues: 92-332; Coverage: 55%)
Gene name: sigA
Sequence domains:
Structure domains: Four Helix Bundle (Hemerythrin (Met), subunit A)
5'-D(*TP*GP*TP*AP*TP*AP*AP*TP*GP*GP*G)-3' Chains: B, C
Molecule details ›
Chains: B, C
Length: 11 nucleotides
Theoretical weight: 3.43 KDa
Source organism: Thermus aquaticus
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P422
Unit cell:
a: 86.001Å b: 86.001Å c: 105.862Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.238
Expression systems:
  • Escherichia coli
  • Not provided