PDBe 3ugd

X-ray diffraction
1.45Å resolution

Structural and functional characterization of an anesthetic binding site in the second cysteine-rich domain of protein kinase C delta

Released:

Function and Biology Details

Reactions catalysed:
ATP + a protein = ADP + a phosphoprotein
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein kinase C delta type regulatory subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 65 amino acids
Theoretical weight: 7.35 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P28867 (Residues: 231-280; Coverage: 7%)
Gene names: Pkcd, Prkcd
Structure domains: Wheat Germ Agglutinin (Isolectin 2); domain 1

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P21
Unit cell:
a: 43.946Å b: 32.504Å c: 49.677Å
α: 90° β: 94.21° γ: 90°
R-values:
R R work R free
0.177 0.173 0.218
Expression system: Escherichia coli