PDBe 3udl

X-ray diffraction
2.17Å resolution

3-heterocyclyl quinolone bound to HCV NS5B

Released:

Function and Biology Details

Reactions catalysed:
ATP + H(2)O = ADP + phosphate
NTP + H(2)O = NDP + phosphate
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 576 amino acids
Theoretical weight: 64.1 KDa
Source organism: Hepatitis C virus subtype 1b
Expression system: Escherichia coli
UniProt:
  • Canonical: P26663 (Residues: 2420-2989; Coverage: 19%)
Sequence domains: Viral RNA dependent RNA polymerase
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 101.746Å b: 101.863Å c: 250.867Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.191 0.243
Expression system: Escherichia coli