PDBe 3ubl

X-ray diffraction
2Å resolution

Crystal structure of glutathione transferase (TARGET EFI-501770) from leptospira interrogans with gsh bound

Released:
Entry authors: Patskovsky Y, Toro R, Bhosle R, Zencheck WD, Hillerich B, Seidel RD, Washington E, Scott Glenn A, Chowdhury S, Evans B, Hammonds J, Imker HJ, Armstrong RN, Gerlt JA, Almo SC, (Efi) Enzyme Function Initiative

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione transferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 242 amino acids
Theoretical weight: 27.72 KDa
Source organism: Leptospira interrogans serovar Lai str. 56601
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8F2Y8 (Residues: 1-218; Coverage: 100%)
Gene names: LA_2623, gst
Sequence domains: Glutathione S-transferase, N-terminal domain
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P4322
Unit cell:
a: 82.591Å b: 82.591Å c: 175.701Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.2 0.235
Expression system: Escherichia coli BL21(DE3)