3uai

X-ray diffraction
3.06Å resolution

Structure of the Shq1-Cbf5-Nop10-Gar1 complex from Saccharomyces cerevisiae

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
H/ACA ribonucleoprotein complex subunit CBF5 Chain: A
Molecule details ›
Chain: A
Length: 400 amino acids
Theoretical weight: 44.98 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P33322 (Residues: 3-394; Coverage: 81%)
Gene names: CBF5, L9470.11, YLR175W
Sequence domains:
Structure domains:
H/ACA ribonucleoprotein complex subunit NOP10 Chain: B
Molecule details ›
Chain: B
Length: 58 amino acids
Theoretical weight: 6.65 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6Q547 (Residues: 1-58; Coverage: 100%)
Gene names: NOP10, YHR072W-A
Sequence domains: Nucleolar RNA-binding protein, Nop10p family
H/ACA ribonucleoprotein complex subunit GAR1 Chain: C
Molecule details ›
Chain: C
Length: 114 amino acids
Theoretical weight: 12.62 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P28007 (Residues: 32-124; Coverage: 45%)
Gene names: GAR1, YHR089C
Structure domains: Probable tRNA pseudouridine synthase domain
Protein SHQ1 Chain: D
Molecule details ›
Chain: D
Length: 366 amino acids
Theoretical weight: 42.93 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P40486 (Residues: 147-507; Coverage: 71%)
Gene names: SHQ1, YIL104C
Sequence domains: SHQ1 protein

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P43212
Unit cell:
a: 100.995Å b: 100.995Å c: 272.329Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.219 0.275
Expression system: Escherichia coli