› 3u8x

X-ray diffraction
2Å resolution

Crystal Structure of a chimera containing the N-terminal domain (residues 8-29) of drosophila Ciboulot and the C-terminal domain (residues 18-44) of bovine Thymosin-beta4, bound to G-actin-ATP

Released:
DOI: 10.2210/pdb3u8x/pdb
PDB entry 3u8x coloured by chain, front view.
PDB entry 3u8x coloured by chain, side view.
PDB entry 3u8x coloured by chain, top view.
Source organisms:
Primary publication:
How a single residue in individual β-thymosin/WH2 domains controls their functions in actin assembly.
Didry D, Cantrelle FX, Husson C, Roblin P, Moorthy AM, Perez J, Le Clainche C, Hertzog M, Guittet E, Carlier MF, van Heijenoort C, Renault L
EMBO J 31 1000-13 (2012)
PMID: 22193718

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132187 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, alpha skeletal muscle Chains: A, C
Molecule details ›
Chains: A, C
Length: 375 amino acids
Theoretical weight: 41.86 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:
Thymosin beta-4; WH2 domain-containing protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 54 amino acids
Theoretical weight: 5.99 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O97428 (Residues: 8-31; Coverage: 19%)
  • Canonical: P62326 (Residues: 20-44; Coverage: 57%)
Gene names: CG4944, Dmel\CG4944, Dmel_CG4944, EG0007-11, EG:EG0007.11, THYB4, TMSB4, cib
Sequence domains: Thymosin beta-4 family

Ligands and Environments

2 bound ligands:
Ligand ATP 2 x ATP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 46.011Å b: 75.326Å c: 128.866Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.186 0.238
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Disordered proteinaceous machines.
Fuxreiter et al. (2014)
9 more

1 mention without citation

How a single residue in individual β-thymosin/WH2 domains controls their functions in actin assembly.
Didry et al. (2012)