› 3u8p

X-ray diffraction
2.75Å resolution

Cytochrome b562 integral fusion with EGFP

Released:
DOI: 10.2210/pdb3u8p/pdb
PDB entry 3u8p coloured by chain, front view.
PDB entry 3u8p coloured by chain, side view.
PDB entry 3u8p coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for efficient chromophore communication and energy transfer in a constructed didomain protein scaffold.
Arpino JA, Czapinska H, Piasecka A, Edwards WR, Barker P, Gajda MJ, Bochtler M, Jones DD
J Am Chem Soc 134 13632-40 (2012)
PMID: 22822710

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142093 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein; Soluble cytochrome b562 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 347 amino acids
Theoretical weight: 38.98 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 2-38, 40-238; Coverage: 99%)
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene names: GFP, cybC
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand HEM 3 x HEM
1 modified residue:
Ligand CRO 3 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 64.752Å b: 125.2Å c: 89.255Å
α: 90° β: 90.37° γ: 90°
R-values:
R R work R free
0.203 0.201 0.242
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks.
Greenwald et al. (2018)
4 more

5 mentions without citation

Heme dynamics and trafficking factors revealed by genetically encoded fluorescent heme sensors.
Hanna et al. (2016)
4 more