PDBe 3u54

X-ray diffraction
2.35Å resolution

Crystal structure (Type-1) of SAICAR synthetase from Pyrococcus horikoshii OT3

Released:

Function and Biology Details

Reaction catalysed:
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo hexamer
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosylaminoimidazole-succinocarboxamide synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 238 amino acids
Theoretical weight: 27.71 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O57978 (Residues: 1-238; Coverage: 100%)
Gene names: PH0239, purC
Sequence domains: SAICAR synthetase
Structure domains:

Ligands and Environments


1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: R3
Unit cell:
a: 95.42Å b: 95.42Å c: 148.631Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.237 0.235 0.286
Expression system: Escherichia coli