3u44

X-ray diffraction
3.2Å resolution

Crystal structure of AddAB-DNA complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (3 distinct):
ATP-dependent helicase/nuclease subunit A Chain: A
ATP-dependent helicase/deoxyribonuclease subunit B Chain: B
Molecule details ›
Chain: B
Length: 1166 amino acids
Theoretical weight: 134.77 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P23477 (Residues: 1-1166; Coverage: 100%)
Gene names: BSU10620, addB
Sequence domains:
Structure domains:
DNA (36-MER) Chain: X
Molecule details ›
Chain: X
Length: 48 nucleotides
Theoretical weight: 14.72 KDa

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 100.23Å b: 138.96Å c: 102.97Å
α: 90° β: 105.33° γ: 90°
R-values:
R R work R free
0.235 0.233 0.284
Expression system: Escherichia coli