3u3w

X-ray diffraction
2.4Å resolution

Crystal Structure of Bacillus thuringiensis PlcR in complex with the peptide PapR7 and DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
HTH cro/C1-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 293 amino acids
Theoretical weight: 35.06 KDa
Source organism: Bacillus thuringiensis Bt407
Expression system: Escherichia coli
UniProt:
  • Canonical: Q45782 (Residues: 1-285; Coverage: 100%)
Gene names: B4918_30015, C6351_13005, CAB88_27845, CN398_18815, COJ78_00750, COK99_05395, CON71_19630, FBF59_20845, FOC94_30660, HKK62_14725, plcR
Sequence domains:
Structure domains:
PlcR associated protein, PapR Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 7 amino acids
Theoretical weight: 838 Da
Source organism: Bacillus cereus
Expression system: Not provided
UniProt:
  • Canonical: B7IR02 (Residues: 42-48; Coverage: 15%)
Gene names: BCG9842_B5480, papR
5'-D(P*CP*TP*AP*TP*GP*CP*AP*AP*TP*AP*TP*TP*TP*CP*AP*TP*AP*T)-3' Chain: Y
Molecule details ›
Chain: Y
Length: 18 nucleotides
Theoretical weight: 5.46 KDa
5'-D(P*AP*TP*AP*TP*GP*AP*AP*AP*TP*AP*TP*TP*GP*CP*AP*TP*AP*G)-3' Chain: Z
Molecule details ›
Chain: Z
Length: 18 nucleotides
Theoretical weight: 5.56 KDa

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 88.88Å b: 71.088Å c: 88.853Å
α: 90° β: 115.53° γ: 90°
R-values:
R R work R free
0.182 0.179 0.235
Expression systems:
  • Escherichia coli
  • Not provided