3u1j

X-ray diffraction
1.8Å resolution

Aprotinin bound to Dengue virus protease

Released:

Function and Biology Details

Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
NTP + H(2)O = NDP + phosphate
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA]
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Serine protease subunit NS2B Chain: A
Molecule details ›
Chain: A
Length: 51 amino acids
Theoretical weight: 5.59 KDa
Source organism: Dengue virus 3 Singapore/8120/1995
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5UB51 (Residues: 1393-1438; Coverage: 1%)
Sequence domains: Flavivirus non-structural protein NS2B
Serine protease NS3 Chain: B
Molecule details ›
Chain: B
Length: 191 amino acids
Theoretical weight: 20.15 KDa
Source organism: Dengue virus 3 Singapore/8120/1995
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5UB51 (Residues: 1474-1655; Coverage: 5%)
Sequence domains: Peptidase S7, Flavivirus NS3 serine protease
Structure domains:
Pancreatic trypsin inhibitor Chain: E
Molecule details ›
Chain: E
Length: 58 amino acids
Theoretical weight: 6.53 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00974 (Residues: 36-93; Coverage: 73%)
Sequence domains: Kunitz/Bovine pancreatic trypsin inhibitor domain
Structure domains: Pancreatic trypsin inhibitor Kunitz domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P321
Unit cell:
a: 84.789Å b: 84.789Å c: 66.031Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.186 0.204
Expression system: Escherichia coli