Function and Biology Details
Reactions catalysed:
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
- not assigned
Cellular component:
Sequence domains:
- Pancreatic trypsin inhibitor Kunitz domain
- Pancreatic trypsin inhibitor Kunitz domain superfamily
- Non-structural protein NS3, peptidase S7, flavivirus
- Non-structural protein NS2B, flavivirus
- Proteinase inhibitor I2, Kunitz, conserved site
- Peptidase S1, PA clan
- Peptidase S1, PA clan, chymotrypsin-like fold
Structure analysis Details
Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133759 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Serine protease subunit NS2B
Molecule details ›
Chain: A
Length: 51 amino acids
Theoretical weight: 5.59 KDa
Source organism: Dengue virus 3 Singapore/8120/1995
Expression system: Escherichia coli
UniProt:
Length: 51 amino acids
Theoretical weight: 5.59 KDa
Source organism: Dengue virus 3 Singapore/8120/1995
Expression system: Escherichia coli
UniProt:
- Canonical:
Q5UB51 (Residues: 1393-1438; Coverage: 1%)
Serine protease NS3
Molecule details ›
Chain: B
Length: 191 amino acids
Theoretical weight: 20.15 KDa
Source organism: Dengue virus 3 Singapore/8120/1995
Expression system: Escherichia coli
UniProt:
Structure domains:
Length: 191 amino acids
Theoretical weight: 20.15 KDa
Source organism: Dengue virus 3 Singapore/8120/1995
Expression system: Escherichia coli
UniProt:
- Canonical: Q5UB51 (Residues: 1474-1655; Coverage: 5%)
Structure domains:
Pancreatic trypsin inhibitor
Molecule details ›
Chain: E
Length: 58 amino acids
Theoretical weight: 6.53 KDa
Source organism: Bos taurus
UniProt:
Structure domains: Pancreatic trypsin inhibitor Kunitz domain
Length: 58 amino acids
Theoretical weight: 6.53 KDa
Source organism: Bos taurus
UniProt:
- Canonical: P00974 (Residues: 36-93; Coverage: 73%)
Structure domains: Pancreatic trypsin inhibitor Kunitz domain
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
SLS BEAMLINE X10SA
Spacegroup:
P321
Expression system: Escherichia coli
