› 3tw2

X-ray diffraction
1.38Å resolution

High resolution structure of human histidine triad nucleotide-binding protein 1 (hHINT1)/AMP complex in a monoclinic space group

Released:
DOI: 10.2210/pdb3tw2/pdb
PDB entry 3tw2 coloured by chain, front view.
PDB entry 3tw2 coloured by chain, side view.
PDB entry 3tw2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A new crystal form of human histidine triad nucleotide-binding protein 1 (hHINT1) in complex with adenosine 5'-monophosphate at 1.38 Å resolution.
Dolot R, Ozga M, Włodarczyk A, Krakowiak A, Nawrot B
Acta Crystallogr Sect F Struct Biol Cryst Commun 68 883-8 (2012)
PMID: 22869114

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156024 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosine 5'-monophosphoramidase HINT1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 126 amino acids
Theoretical weight: 13.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49773 (Residues: 1-126; Coverage: 100%)
Gene names: HINT, HINT1, PKCI1, PRKCNH1
Sequence domains: HIT domain
Structure domains: HIT-like

Ligands and Environments

1 bound ligand:
Ligand AMP 1 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: C2
Unit cell:
a: 77.421Å b: 46.449Å c: 64.034Å
α: 90° β: 94.42° γ: 90°
R-values:
R R work R free
0.123 0.121 0.161
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Molecular underpinnings of Aprataxin RNA/DNA deadenylase function and dysfunction in neurological disease.
Schellenberg et al. (2015)
1 more

5 mentions without citation

A new crystal form of human histidine triad nucleotide-binding protein 1 (hHINT1) in complex with adenosine 5'-monophosphate at 1.38 Å resolution.
Dolot et al. (2012)
4 more