3tq6

X-ray diffraction
2.45Å resolution

Crystal structure of human mitochondrial transcription factor A, TFAM or mtTFA, bound to the light strand promoter LSP

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcription factor A, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 214 amino acids
Theoretical weight: 25.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q00059 (Residues: 43-246; Coverage: 83%)
Gene names: TCF6, TCF6L2, TFAM
Sequence domains:
Structure domains: High mobility group box domain
DNA (5'-D(*TP*AP*AP*CP*AP*GP*TP*CP*AP*CP*CP*CP*CP*CP*CP*AP*AP*CP*(BRU)P*AP*AP*C)-3') Chains: C, E
Molecule details ›
Chains: C, E
Length: 22 nucleotides
Theoretical weight: 6.66 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*GP*TP*TP*AP*GP*TP*TP*GP*GP*GP*GP*GP*GP*TP*GP*AP*CP*TP*GP*TP*TP*A)-3') Chains: D, F
Molecule details ›
Chains: D, F
Length: 22 nucleotides
Theoretical weight: 6.91 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21212
Unit cell:
a: 113.9Å b: 117.2Å c: 56.53Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.182 0.228
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided