3tmm

X-ray diffraction
2.5Å resolution

TFAM imposes a U-turn on mitochondrial DNA

Released:
Source organism: Homo sapiens
Primary publication:
The mitochondrial transcription and packaging factor Tfam imposes a U-turn on mitochondrial DNA.
OpenAccess logo Nat. Struct. Mol. Biol. 18 1290-6 (2011)
PMID: 22037171

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcription factor A, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 238 amino acids
Theoretical weight: 28.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q00059 (Residues: 43-246; Coverage: 83%)
Gene names: TCF6, TCF6L2, TFAM
Sequence domains:
Structure domains: High mobility group box domain
DNA (28-MER) Chain: B
Molecule details ›
Chain: B
Length: 28 nucleotides
Theoretical weight: 8.79 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (28-MER) Chain: C
Molecule details ›
Chain: C
Length: 28 nucleotides
Theoretical weight: 8.42 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: C2221
Unit cell:
a: 68.438Å b: 81.911Å c: 161.254Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.248
Expression systems:
  • Escherichia coli BL21
  • Not provided