X-ray diffraction
3.01Å resolution

Crystal structure and RNA binding properties of the RRM/AlkB domains in human ABH8, an enzyme catalyzing tRNA hypermodification, Northeast Structural Genomics Consortium Target HR5601B


Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + carboxymethyluridine(34) in tRNA = S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine(34) in tRNA
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
homo dimer
Entry contents:
1 distinct polypeptide molecule
Alkylated DNA repair protein alkB homolog 8 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 345 amino acids
Theoretical weight: 39.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: Q96BT7 (Residues: 25-355; Coverage: 50%)
Gene names: ABH8, ALKBH8
Sequence domains: 2OG-Fe(II) oxygenase superfamily
Structure domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 150.166Å b: 73.279Å c: 149.814Å
α: 90° β: 112.73° γ: 90°
R R work R free
0.224 0.22 0.277
Expression system: Escherichia coli