› 3tck

X-ray diffraction
2.3Å resolution

Crystal structure of Peptidyl-tRNA hydrolase from Mycobacterium tuberculosis - Form 4

Released:
DOI: 10.2210/pdb3tck/pdb
PDB entry 3tck coloured by chain, front view.
PDB entry 3tck coloured by chain, side view.
PDB entry 3tck coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structures of new crystal forms of Mycobacterium tuberculosis peptidyl-tRNA hydrolase and functionally important plasticity of the molecule.
Selvaraj M, Ahmad R, Varshney U, Vijayan M
Acta Crystallogr Sect F Struct Biol Cryst Commun 68 124-8 (2012)
PMID: 22297982

Function and Biology Details

Reaction catalysed: 
N-substituted aminoacyl-tRNA + H(2)O = N-substituted amino acid + tRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161572 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidyl-tRNA hydrolase Chain: A
Molecule details ›
Chain: A
Length: 191 amino acids
Theoretical weight: 20.49 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHN7 (Residues: 1-191; Coverage: 100%)
Gene names: MTCY10G2.35, Rv1014c, pth
Sequence domains: Peptidyl-tRNA hydrolase
Structure domains: Peptidyl-tRNA hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 36.3Å b: 38.81Å c: 122.88Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.268
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

A tRNA-Acetylating Toxin and Detoxifying Enzyme in Mycobacterium tuberculosis.
Tomasi et al. (2022)
1 more

8 mentions without citation

A single regulatory gene is sufficient to alter bacterial host range.
Mandel et al. (2009)
7 more