3t4a

X-ray diffraction
3.4Å resolution

Structure of a truncated form of Staphylococcal Complement Inhibitor B bound to human C3c at 3.4 Angstrom resolution

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Complement C3 beta chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 645 amino acids
Theoretical weight: 71.39 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01024 (Residues: 23-667; Coverage: 39%)
Gene names: C3, CPAMD1
Sequence domains:
Structure domains:
Complement C3c alpha' chain fragment 1 Chains: B, E
Molecule details ›
Chains: B, E
Length: 206 amino acids
Theoretical weight: 23.62 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01024 (Residues: 749-954; Coverage: 13%)
Gene names: C3, CPAMD1
Sequence domains: Alpha-2-macroglobulin family
Structure domains:
Complement C3c alpha' chain fragment 2 Chains: C, F
Molecule details ›
Chains: C, F
Length: 343 amino acids
Theoretical weight: 39.54 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01024 (Residues: 1321-1663; Coverage: 21%)
Gene names: C3, CPAMD1
Sequence domains:
Structure domains:
Fibrinogen-binding protein Chains: G, H
Molecule details ›
Chains: G, H
Length: 73 amino acids
Theoretical weight: 8.21 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu50
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3JZ23 (Residues: 49-116; Coverage: 80%)
Gene name: SAV1159
Structure domains: Substrate Binding Domain Of Dnak; Chain:A; Domain 2

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P212121
Unit cell:
a: 103.304Å b: 165.436Å c: 203.125Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.229 0.27
Expression system: Escherichia coli BL21(DE3)