3szq

X-ray diffraction
2.35Å resolution

Structure of an S. pombe APTX/DNA/AMP/Zn complex

Released:

Function and Biology Details

Reactions catalysed:
(DNA)-3'-diphospho-5'-guanosine + H(2)O = (DNA)-3'-phosphate + GMP
Adenosine-5'-diphospho-5'-(DNA) + H(2)O = AMP + phospho-5'-(DNA)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Aprataxin-like protein Chain: A
Molecule details ›
Chain: A
Length: 206 amino acids
Theoretical weight: 23.9 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli
UniProt:
  • Canonical: O74859 (Residues: 31-232; Coverage: 87%)
Gene names: SPCC18.09c, hnt3
Sequence domains:
Structure domains: HIT-like
5'-D(*CP*CP*CP*TP*G)-3' Chain: B
Molecule details ›
Chain: B
Length: 5 nucleotides
Theoretical weight: 1.46 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Not provided
5'-D(*TP*AP*TP*CP*GP*GP*AP*AP*TP*CP*AP*GP*GP*G)-3' Chain: C
Molecule details ›
Chain: C
Length: 14 nucleotides
Theoretical weight: 4.34 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Not provided

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: I432
Unit cell:
a: 157.05Å b: 157.05Å c: 157.05Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.165 0.211
Expression systems:
  • Escherichia coli
  • Not provided