3svv

X-ray diffraction
2.2Å resolution

Crystal Structure of T338C c-Src covalently bound to vinylsulfonamide-pyrazolopyrimidine 9

Released:
Source organism: Gallus gallus
Primary publication:
Chemical genetic strategy for targeting protein kinases based on covalent complementarity.
Proc. Natl. Acad. Sci. U.S.A. 108 15046-52 (2011)
PMID: 21852571

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proto-oncogene tyrosine-protein kinase Src Chains: A, B
Molecule details ›
Chains: A, B
Length: 286 amino acids
Theoretical weight: 32.73 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00523 (Residues: 251-533; Coverage: 53%)
Gene name: SRC
Sequence domains: Protein tyrosine kinase
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P1
Unit cell:
a: 42.3Å b: 63.315Å c: 74.23Å
α: 78.93° β: 87.7° γ: 87.92°
R-values:
R R work R free
0.178 0.176 0.215
Expression system: Escherichia coli BL21(DE3)