3suo

X-ray diffraction
2.23Å resolution

RB69 DNA Polymerase (Y567A) Ternary Complex with dTTP Opposite 2AP (GC rich sequence)

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-directed DNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 900 amino acids
Theoretical weight: 104.15 KDa
Source organism: Enterobacteria phage RB69
Expression system: Escherichia coli
UniProt:
  • Canonical: Q38087 (Residues: 1-900; Coverage: 100%)
Gene name: 43
Sequence domains:
Structure domains:
5'-D(P*CP*(2PR)P*TP*CP*GP*CP*CP*GP*CP*CP*GP*CP*GP*CP*GP*G)-3' Chain: T
Molecule details ›
Chain: T
Length: 16 nucleotides
Theoretical weight: 4.86 KDa
Source organism: Escherichia phage RB69
Expression system: Not provided
5'-D(*CP*GP*CP*GP*CP*GP*GP*CP*GP*GP*CP*GP*(2DA))-3' Chain: P
Molecule details ›
Chain: P
Length: 13 nucleotides
Theoretical weight: 4 KDa
Source organism: Escherichia phage RB69
Expression system: Not provided

Ligands and Environments

2 bound ligands:

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 74.401Å b: 120.073Å c: 130.885Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.207 0.255
Expression systems:
  • Escherichia coli
  • Not provided