X-ray diffraction
4.39Å resolution

Crystal structure of the intracellular domain of Plexin-B1 in complex with Rac1

Source organism: Homo sapiens
Primary publication:
A dual binding mode for RhoGTPases in plexin signalling.
OpenAccess logo PLoS Biol 9 e1001134 (2011)
PMID: 21912513

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero hexamer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ras-related C3 botulinum toxin substrate 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 184 amino acids
Theoretical weight: 20.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P63000 (Residues: 1-177; Coverage: 92%)
Gene names: MIG5, RAC1, TC25
Plexin-B1 Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 633 amino acids
Theoretical weight: 72.63 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
  • Canonical: O43157 (Residues: 1511-2135; Coverage: 30%)
Gene names: KIAA0407, PLXN5, PLXNB1, SEP
Sequence domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: I212121
Unit cell:
a: 142.34Å b: 224.25Å c: 258.16Å
α: 90° β: 90° γ: 90°
R R work R free
0.235 0.234 0.264
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda