› 3sua

X-ray diffraction
4.39Å resolution

Crystal structure of the intracellular domain of Plexin-B1 in complex with Rac1

Released:
DOI: 10.2210/pdb3sua/pdb
PDB 3sua coloured by chain and viewed from the front.
PDB 3sua coloured by chain and viewed from the side.
PDB 3sua coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
A dual binding mode for RhoGTPases in plexin signalling.
Bell CH, Aricescu AR, Jones EY, Siebold C
OpenAccess logo PLoS Biol 9 e1001134 (2011)
PMID: 21912513

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero hexamer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ras-related C3 botulinum toxin substrate 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 184 amino acids
Theoretical weight: 20.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63000 (Residues: 1-177; Coverage: 92%)
Gene names: MIG5, RAC1, TC25
Plexin-B1 Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 633 amino acids
Theoretical weight: 72.63 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O43157 (Residues: 1511-2135; Coverage: 30%)
Gene names: KIAA0407, PLXN5, PLXNB1, SEP
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand MG 3 x MG
Ligand GNP 3 x GNP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: I212121
Unit cell:
a: 142.34Å b: 224.25Å c: 258.16Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.234 0.264
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda

Quick links

Citations

9 review citations

Allostery and dynamics in small G proteins.
Mott et al. (2018)
8 more

2 mentions without citation

Structural mechanisms of plexin signaling.
Pascoe et al. (2015)
1 more