3sse

X-ray diffraction
2.7Å resolution

DNA binding domain of restriction endonuclease bound to DNA

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
5-methylcytosine-specific restriction enzyme B Chains: A, B
Molecule details ›
Chains: A, B
Length: 170 amino acids
Theoretical weight: 19.66 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P15005 (Residues: 1-161; Coverage: 35%)
Gene names: JW5871, b4346, mcrB, rglB
Sequence domains: MrcB-like, N-terminal domain
Structure domains: Metal Transport, Frataxin; Chain A
DNA (5'-D(*T*GP*AP*GP*AP*CP*CP*GP*GP*TP*AP*GP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 13 nucleotides
Theoretical weight: 4.02 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided
DNA (5'-D(*A*GP*CP*TP*AP*CP*CP*GP*GP*TP*CP*TP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 13 nucleotides
Theoretical weight: 3.93 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P212121
Unit cell:
a: 36.046Å b: 69.087Å c: 143.896Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.216 0.292
Expression systems:
  • Escherichia coli
  • Not provided