3sja

X-ray diffraction
3Å resolution

Crystal structure of S. cerevisiae Get3 in the open state in complex with Get1 cytosolic domain

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ATPase GET3 Chains: A, B, E, F, I
Molecule details ›
Chains: A, B, E, F, I
Length: 362 amino acids
Theoretical weight: 40.46 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12154 (Residues: 1-354; Coverage: 100%)
Gene names: ARR4, D2371, GET3, YDL100C
Sequence domains: Anion-transporting ATPase
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Golgi to ER traffic protein 1 Chains: C, D, G, H, J
Molecule details ›
Chains: C, D, G, H, J
Length: 65 amino acids
Theoretical weight: 7.9 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P53192 (Residues: 36-93; Coverage: 25%)
Gene names: GET1, MDM39, YGL020C
Structure domains: Helix hairpin bin

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21212
Unit cell:
a: 204.12Å b: 91.46Å c: 149.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.231
Expression system: Escherichia coli BL21(DE3)