› 3sj0

X-ray diffraction
2Å resolution

PpcA mutant M58S

Released:
DOI: 10.2210/pdb3sj0/pdb
PDB entry 3sj0 coloured by chain, front view.
PDB entry 3sj0 coloured by chain, side view.
PDB entry 3sj0 coloured by chain, top view.
Source organism: Geobacter sulfurreducens
Primary publication:
Pitfalls in the interpretation of structural changes in mutant proteins from crystal structures.
Pokkuluri PR, Yang X, Londer YY, Schiffer M
J Struct Funct Genomics 13 227-32 (2012)
PMID: 23099666

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-184510 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PpcA Chain: X
Molecule details ›
Chain: X
Length: 71 amino acids
Theoretical weight: 7.7 KDa
Source organism: Geobacter sulfurreducens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8GGK7 (Residues: 21-91; Coverage: 100%)
Gene name: ppcA
Sequence domains: Cytochrome c7 and related cytochrome c
Structure domains: Cytochrome C3

Ligands and Environments

3 bound ligands:
Ligand HEC 3 x HEC
Ligand DXC 1 x DXC
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P4322
Unit cell:
a: 32.4Å b: 32.4Å c: 178.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.19 0.22
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Molecular interactions between Geobacter sulfurreducens triheme cytochromes and the electron acceptor Fe(iii) citrate studied by NMR.
Ferreira et al. (2017)

1 mention without citation

Pitfalls in the interpretation of structural changes in mutant proteins from crystal structures.
Pokkuluri et al. (2012)