3siv

X-ray diffraction
3.3Å resolution

Structure of a hPrp31-15.5K-U4atac 5' stem loop complex, dimeric form

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
NHP2-like protein 1 Chains: A, D, G, J
Molecule details ›
Chains: A, D, G, J
Length: 130 amino acids
Theoretical weight: 14.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P55769 (Residues: 1-128; Coverage: 100%)
Gene names: NHP2L1, SNU13
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Structure domains: 60s Ribosomal Protein L30; Chain: A;
U4/U6 small nuclear ribonucleoprotein Prp31 Chains: B, E, H, K
Molecule details ›
Chains: B, E, H, K
Length: 254 amino acids
Theoretical weight: 28.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8WWY3 (Residues: 85-333; Coverage: 50%)
  • Best match: Q8WWY3-3 (Residues: 5-253)
Gene names: PRP31, PRPF31
Sequence domains: snoRNA binding domain, fibrillarin
Structure domains: Serum Albumin; Chain A, Domain 1
U4atac snRNA Chains: C, F, I, L
Molecule details ›
Chains: C, F, I, L
Length: 32 nucleotides
Theoretical weight: 10.34 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2
Unit cell:
a: 255.407Å b: 105.325Å c: 188.644Å
α: 90° β: 127.52° γ: 90°
R-values:
R R work R free
0.2 0.198 0.239
Expression system: Escherichia coli