3sho

X-ray diffraction
1.8Å resolution

Crystal structure of RpiR transcription factor from Sphaerobacter thermophilus (sugar isomerase domain)

Released:
Entry authors: Michalska K, Tesar C, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulator, RpiR family Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 187 amino acids
Theoretical weight: 20.12 KDa
Source organism: Sphaerobacter thermophilus DSM 20745
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: D1C9F1 (Residues: 96-282; Coverage: 65%)
Gene name: Sthe_3042
Sequence domains: SIS domain
Structure domains: Glucose-6-phosphate isomerase like protein; domain 1

Ligands and Environments

No bound ligands
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21
Unit cell:
a: 68.194Å b: 77.816Å c: 71.157Å
α: 90° β: 94.89° γ: 90°
R-values:
R R work R free
0.174 0.173 0.209
Expression system: Escherichia coli BL21