PDBe 3sg3

X-ray diffraction
2.1Å resolution

Function and Biology Details

Reaction catalysed:
ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calmodulin-1; Green fluorescent protein; Myosin light chain kinase, smooth muscle, deglutamylated form Chain: A
Molecule details ›
Chain: A
Length: 449 amino acids
Theoretical weight: 50.75 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P11799 (Residues: 1731-1749, 1752-1763; Coverage: 2%)
  • Canonical: P42212 (Residues: 2-144, 151-170, 191-192; Coverage: 69%)
  • Canonical: P0DP29 (Residues: 23-42, 45-149; Coverage: 84%)
Gene names: CaMI, Calm, Calm1, Cam, Cam1, GFP, Mylk
Sequence domains: EF-hand domain pair
Structure domains:

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P41212
Unit cell:
a: 120.444Å b: 120.444Å c: 98Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.165 0.199
Expression system: Escherichia coli