3sck

X-ray diffraction
3Å resolution

Crystal structure of spike protein receptor-binding domain from a predicted SARS coronavirus civet strain complexed with human-civet chimeric receptor ACE2

Released:
Source organisms:
Entry authors: Wu K, Peng G, Wilken M, Geraghty R, Li F

Function and Biology Details

Reaction catalysed:
Angiotensin II + H(2)O = angiotensin-(1-7) + L-phenylalanine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Processed angiotensin-converting enzyme 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 603 amino acids
Theoretical weight: 69.88 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q56NL1 (Residues: 21-55, 82-85; Coverage: 5%)
  • Canonical: Q9BYF1 (Residues: 19-20, 56-81, 86-615; Coverage: 71%)
Gene names: ACE2, UNQ868/PRO1885
Sequence domains: Angiotensin-converting enzyme
Spike protein S1 Chains: E, F
Molecule details ›
Chains: E, F
Length: 185 amino acids
Theoretical weight: 21.22 KDa
UniProt:
  • Canonical: P59594 (Residues: 324-502; Coverage: 14%)
Gene names: 2, S
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 81.219Å b: 119.277Å c: 113.236Å
α: 90° β: 92.19° γ: 90°
R-values:
R R work R free
0.242 0.239 0.285
Expression system: Spodoptera frugiperda