PDBe 3sb8

X-ray diffraction
2.65Å resolution

Cu-mediated Dimer of T4 Lysozyme D61H/K65H by Synthetic Symmetrization

Released:

Function and Biology Details

Reaction catalysed:
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
monomeric
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 165 amino acids
Theoretical weight: 18.64 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P00720 (Residues: 1-162; Coverage: 99%)
Gene name: E
Sequence domains: Phage lysozyme
Structure domains: Lysozyme

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 44.88Å b: 95.18Å c: 117.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.222 0.273
Expression system: Escherichia coli