3rwe

X-ray diffraction
2.4Å resolution

Function and Biology Details

Reactions catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 32.03 KDa
Source organism: Macaca mulatta
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9GJ77 (Residues: 24-299; Coverage: 80%)
Gene name: MHCI-B
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.66 KDa
Source organism: Macaca mulatta
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6V7J5 (Residues: 21-119; Coverage: 100%)
Gene name: B2M
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Pol protein Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.26 KDa
Source organism: Simian immunodeficiency virus
Expression system: Not provided
UniProt:
  • Canonical: Q5QGH9 (Residues: 435-443; Coverage: 1%)
Gene name: pol

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P2
Unit cell:
a: 68.808Å b: 45.29Å c: 81.982Å
α: 90° β: 96.11° γ: 90°
R-values:
R R work R free
0.206 0.203 0.251
Expression systems:
  • Escherichia coli
  • Not provided