PDBe 3rto

X-ray diffraction
1.8Å resolution

Acoustically mounted porcine insulin microcrystals yield an X-ray SAD structure

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero 12-mer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P01315 (Residues: 88-108; Coverage: 25%)
Gene name: INS
Insulin B chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 30 amino acids
Theoretical weight: 3.4 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P01315 (Residues: 25-54; Coverage: 36%)
Gene name: INS

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B, NSLS BEAMLINE X12C, NSLS BEAMLINE X25, APS BEAMLINE 23-ID-D
Spacegroup: R3
Unit cell:
a: 81.7Å b: 81.7Å c: 33.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.181 0.223