3rkq

X-ray diffraction
1.7Å resolution

NKX2.5 Homeodomain dimer bound to ANF-242 DNA

Released:
Source organism: Homo sapiens

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Homeobox protein Nkx-2.5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 58 amino acids
Theoretical weight: 7.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P52952 (Residues: 138-194; Coverage: 18%)
Gene names: CSX, NKX2-5, NKX2.5, NKX2E
Structure domains: Homeodomain-like
ANF-242 DNA Chain: C
Molecule details ›
Chain: C
Length: 19 nucleotides
Theoretical weight: 5.92 KDa
Source organism: Homo sapiens
Expression system: Not provided
ANF-242 DNA Chain: D
Molecule details ›
Chain: D
Length: 19 nucleotides
Theoretical weight: 5.73 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P65
Unit cell:
a: 71.45Å b: 71.45Å c: 94.33Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.211 0.257
Expression systems:
  • Escherichia coli
  • Not provided