3ri4

X-ray diffraction
3Å resolution

Ets1 cooperative binding to widely separated sites on promoter DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Protein C-ets-1 Chains: A, D
Molecule details ›
Chains: A, D
Length: 163 amino acids
Theoretical weight: 18.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14921 (Residues: 280-441; Coverage: 37%)
Gene names: ETS1, EWSR2
Sequence domains: Ets-domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
TCR alpha promoter DNA Chains: B, E
Molecule details ›
Chains: B, E
Length: 16 nucleotides
Theoretical weight: 4.84 KDa
TCR alpha promoter DNA Chains: C, F
Molecule details ›
Chains: C, F
Length: 16 nucleotides
Theoretical weight: 4.95 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 50.177Å b: 98.124Å c: 53.577Å
α: 90° β: 109.67° γ: 90°
R-values:
R R work R free
0.23 0.23 0.284
Expression system: Escherichia coli BL21(DE3)