X-ray diffraction
1.61Å resolution

Shigella IpaA-VBS3 in complex with human vinculin

Source organisms:
Primary publication:
Novel vinculin binding site of the IpaA invasin of Shigella.
J. Biol. Chem. 286 23214-21 (2011)
PMID: 21525010

Function and Biology Details

Structure analysis Details

Assemblies composition:
Non-polymer only dimer (preferred)
Non-polymer only tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Vinculin Chains: A, B
Molecule details ›
Chains: A, B
Length: 258 amino acids
Theoretical weight: 28.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P18206 (Residues: 1-258; Coverage: 23%)
Gene name: VCL
Structure domains: Alpha-catenin/vinculin-like
Invasin IpaA Chains: C, D
Molecule details ›
Chains: C, D
Length: 29 amino acids
Theoretical weight: 3.14 KDa
Source organism: Shigella flexneri
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P18010 (Residues: 488-512; Coverage: 4%)
Gene names: CP0125, ipaA
Sequence domains: SipA vinculin binding site

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 53.583Å b: 85.319Å c: 137.904Å
α: 90° β: 90° γ: 90°
R R work R free
0.184 0.184 0.195
Expression system: Escherichia coli BL21(DE3)