PDBe 3reb

X-ray diffraction
3.45Å resolution

HIV-1 Nef protein in complex with engineered Hck-SH3 domain


Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
C-terminal core protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 166 amino acids
Theoretical weight: 19.12 KDa
Source organism: HIV-1 M:B_ARV2/SF2
Expression system: Escherichia coli
  • Canonical: P03407 (Residues: 45-210; Coverage: 79%)
Gene name: nef
Structure domains: Nef Regulatory Factor
Tyrosine-protein kinase HCK Chains: B, D
Molecule details ›
Chains: B, D
Length: 90 amino acids
Theoretical weight: 10.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P08631 (Residues: 79-138; Coverage: 11%)
Gene name: HCK
Sequence domains: SH3 domain
Structure domains: SH3 Domains

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P61
Unit cell:
a: 112.46Å b: 112.46Å c: 130.03Å
α: 90° β: 90° γ: 120°
R R work R free
0.217 0.216 0.243
Expression system: Escherichia coli