3rdt

X-ray diffraction
2.7Å resolution

Crystal Structure of 809.B5 TCR complexed with MHC Class II I-Ab/3k peptide

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
H-2 class II histocompatibility antigen, A-B alpha chain Chain: C
Molecule details ›
Chain: C
Length: 182 amino acids
Theoretical weight: 20.6 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P14434 (Residues: 27-208; Coverage: 78%)
Gene name: H2-Aa
Sequence domains:
Structure domains:
H-2 class II histocompatibility antigen, A beta chain Chain: D
Molecule details ›
Chain: D
Length: 217 amino acids
Theoretical weight: 24.97 KDa
Source organism: synthetic construct
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P14483 (Residues: 21-218; Coverage: 77%)
Gene names: H2-Ab1, H2-iabeta
Sequence domains:
Structure domains:
TCR 809.B5 alpha chain Chain: A
Molecule details ›
Chain: A
Length: 205 amino acids
Theoretical weight: 23.03 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
TCR 809.B5 beta chain Chain: B
Molecule details ›
Chain: B
Length: 241 amino acids
Theoretical weight: 27 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2
Unit cell:
a: 239.855Å b: 73.016Å c: 65.669Å
α: 90° β: 90.71° γ: 90°
R-values:
R R work R free
0.208 0.206 0.241
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli