PDBe 3qsy

X-ray diffraction
3.2Å resolution

Recognition of the methionylated initiator tRNA by the translation initiation factor 2 in Archaea

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
Translation initiation factor 2 subunit gamma Chain: A
Molecule details ›
Chain: A
Length: 415 amino acids
Theoretical weight: 45.85 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q980A5 (Residues: 1-415; Coverage: 100%)
Gene names: SSO0412, eif2g
Sequence domains:
Structure domains:
Translation initiation factor 2 subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 89 amino acids
Theoretical weight: 9.89 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97Z79 (Residues: 176-264; Coverage: 34%)
Gene names: SSO1050, aif2a, eif2a
Structure domains: EIF_2_alpha
tRNA Chain: D
Molecule details ›
Chain: D
Length: 77 nucleotides
Theoretical weight: 24.79 KDa
Sequence domains: tRNA

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P3121
Unit cell:
a: 93.68Å b: 93.68Å c: 220.03Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.279 0.278 0.302
Expression system: Escherichia coli