3qrf

X-ray diffraction
2.8Å resolution

Structure of a domain-swapped FOXP3 dimer

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Nuclear factor of activated T-cells, cytoplasmic 2 Chains: M, N
Molecule details ›
Chains: M, N
Length: 286 amino acids
Theoretical weight: 32.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13469 (Residues: 396-678; Coverage: 31%)
Gene names: NFAT1, NFATC2, NFATP
Sequence domains: Rel homology DNA-binding domain
Structure domains:
Forkhead box protein P3 41 kDa form Chains: F, G, H, I
Molecule details ›
Chains: F, G, H, I
Length: 82 amino acids
Theoretical weight: 10.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BZS1 (Residues: 336-417; Coverage: 19%)
Gene names: FOXP3, IPEX, JM2
Sequence domains: Forkhead domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
human hARRE2 DNA (Plus Strand) Chains: A, C
Molecule details ›
Chains: A, C
Length: 21 nucleotides
Theoretical weight: 6.49 KDa
human hARRE2 DNA (Minus Strand) Chains: B, D
Molecule details ›
Chains: B, D
Length: 21 nucleotides
Theoretical weight: 6.39 KDa

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 83.667Å b: 131.234Å c: 68.665Å
α: 90° β: 89.95° γ: 90°
R-values:
R R work R free
0.248 0.248 0.283
Expression system: Escherichia coli