3qol

X-ray diffraction
1.9Å resolution

Crystal structure of Staphylococcal nuclease variant D+PHS/V23E at pH 6 determined at 100 K

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thermonuclease Chain: A
Molecule details ›
Chain: A
Length: 143 amino acids
Theoretical weight: 16.17 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NXI6 (Residues: 80-228; Coverage: 70%)
Gene names: MW0769, nuc
Sequence domains: Staphylococcal nuclease homologue
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 31.033Å b: 60.453Å c: 37.626Å
α: 90° β: 94.44° γ: 90°
R-values:
R R work R free
0.189 0.187 0.227
Expression system: Escherichia coli