PDBe 3q8m

X-ray diffraction
2.6Å resolution

Crystal Structure of Human Flap Endonuclease FEN1 (D181A) in complex with substrate 5'-flap DNA and K+

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
Flap endonuclease 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 341 amino acids
Theoretical weight: 38.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P39748 (Residues: 2-336; Coverage: 88%)
Gene names: FEN1, RAD2
Sequence domains: XPG I-region
Structure domains:
DNA (5'-D(*AP*CP*TP*CP*TP*GP*CP*CP*TP*CP*AP*AP*GP*AP*CP*GP*GP*T)-3') Chains: D, G
Molecule details ›
Chains: D, G
Length: 18 nucleotides
Theoretical weight: 5.48 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*TP*TP*GP*AP*GP*GP*CP*AP*GP*AP*GP*T)-3') Chains: E, H
Molecule details ›
Chains: E, H
Length: 12 nucleotides
Theoretical weight: 3.74 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*AP*CP*CP*GP*TP*CP*C)-3') Chains: F, I
Molecule details ›
Chains: F, I
Length: 7 nucleotides
Theoretical weight: 2.06 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: P21
Unit cell:
a: 59.52Å b: 94.94Å c: 104.52Å
α: 90° β: 105.06° γ: 90°
R-values:
R R work R free
0.22 0.217 0.272
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided