X-ray diffraction
2.6Å resolution

Crystal structure of the PUMILIO-homology domain from Human PUMILIO1 in complex with hunchback NRE

Source organism: Homo sapiens
Primary publication:
Alternate modes of cognate RNA recognition by human PUMILIO proteins.
Structure 19 361-7 (2011)
PMID: 21397187

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Pumilio homolog 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 349 amino acids
Theoretical weight: 40.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q14671 (Residues: 828-1176; Coverage: 29%)
Gene names: KIAA0099, PUM1, PUMH1
Sequence domains: Pumilio-family RNA binding repeat
Structure domains: Leucine-rich Repeat Variant
5'-R(UP*GP*UP*AP*UP*AP*UP*A)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 8 nucleotides
Theoretical weight: 2.51 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 262.326Å b: 37.483Å c: 82.299Å
α: 90° β: 102.83° γ: 90°
R R work R free
0.196 0.193 0.26
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided