3q0f

X-ray diffraction
2.75Å resolution

Crystal structure of SUVH5 SRA- methylated CHH DNA complex

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Histone-lysine N-methyltransferase, H3 lysine-9 specific SUVH5 Chains: A, X
Molecule details ›
Chains: A, X
Length: 167 amino acids
Theoretical weight: 18.52 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O82175 (Residues: 362-528; Coverage: 21%)
Gene names: At2g35160, SDG9, SET9, SUVH5, T4C15.17
Sequence domains: SAD/SRA domain
Structure domains: SRA-YDG
DNA (5'-D(*CP*TP*GP*AP*GP*GP*AP*GP*TP*AP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.41 KDa
Source organism: Arabidopsis thaliana
Expression system: Not provided
DNA (5'-D(*TP*AP*CP*TP*(5CM)P*CP*TP*CP*AP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 10 nucleotides
Theoretical weight: 2.99 KDa
Source organism: Arabidopsis thaliana
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P6122
Unit cell:
a: 102.968Å b: 102.968Å c: 170.493Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.229 0.273
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided