› 3pi9

X-ray diffraction
2.9Å resolution

Site-specific Glycosylation of Hemoglobin Utilizing Oxime Ligation Chemistry as a Viable Alternative to PEGylation

Released:
DOI: 10.2210/pdb3pi9/pdb
PDB entry 3pi9 coloured by chain, front view.
PDB entry 3pi9 coloured by chain, side view.
PDB entry 3pi9 coloured by chain, top view.
Source organism: Bos taurus
Entry authors: Bhatt VS, Styslinger TJ, Zhang N, Wang PG, Palmer AF

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135382 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hemoglobin subunit alpha Chains: A, C
Molecule details ›
Chains: A, C
Length: 141 amino acids
Theoretical weight: 15.08 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P01966 (Residues: 2-142; Coverage: 99%)
Gene name: HBA
Sequence domains: Globin
Structure domains: Globins
Hemoglobin subunit beta Chains: B, D
Molecule details ›
Chains: B, D
Length: 145 amino acids
Theoretical weight: 15.98 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P02070 (Residues: 1-145; Coverage: 100%)
Gene name: HBB
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

2 bound ligands:
Ligand HEM 4 x HEM
Ligand CMO 4 x CMO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 58.18Å b: 74.01Å c: 131.77Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.263 0.261 0.293

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