PDBe 3p75

X-ray diffraction
1.9Å resolution

Crystal structure of Staphylococcal nuclease variant Delta+PHS V104D at cryogenic temperature

Source organism: Staphylococcus aureus
Entry authors: Khangulov VS, Schlessman JL, Heroux A, Garcia-Moreno EB

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Nuclease A Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.72 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
  • Best match: P00644 (Residues: 89-223)
Gene name: nuc

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 31Å b: 60.791Å c: 36.838Å
α: 90° β: 93.45° γ: 90°
R R work R free
0.189 0.187 0.239
Expression system: Escherichia coli