3p52

X-ray diffraction
2.74Å resolution

NH3-dependent NAD synthetase from Campylobacter jejuni subsp. jejuni NCTC 11168 in complex with the nitrate ion

Released:
Source organism: Campylobacter jejuni
Entry authors: Filippova EV, Wawrzak Z, Onopriyenko O, Skarina T, Edwards A, Savchenko A, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed:
ATP + deamido-NAD(+) + NH(3) = AMP + diphosphate + NAD(+)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo hexamer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NH(3)-dependent NAD(+) synthetase Chains: A, B
Molecule details ›
Chains: A, B
Length: 249 amino acids
Theoretical weight: 28.09 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9PPB0 (Residues: 1-246; Coverage: 100%)
Gene names: Cj0810, nadE
Sequence domains: NAD synthase
Structure domains: HUPs

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P321
Unit cell:
a: 120.945Å b: 120.945Å c: 91.795Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.209 0.245
Expression system: Escherichia coli BL21(DE3)