3p4x

X-ray diffraction
2.41Å resolution

Helicase domain of reverse gyrase from Thermotoga maritima

Released:

Function and Biology Details

Reaction catalysed:
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Reverse gyrase Chains: A, B
Molecule details ›
Chains: A, B
Length: 413 amino acids
Theoretical weight: 48.09 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: O51934 (Residues: 59-541; Coverage: 37%)
Gene names: TM_0173, rgy, topR
Sequence domains: DEAD/DEAH box helicase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 59.259Å b: 111.231Å c: 129.726Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.186 0.256
Expression system: Escherichia coli