3p1s

X-ray diffraction
1.65Å resolution

Crystal structure of human 14-3-3 sigma C38N/N166H in complex with TASK-3 peptide and stabilizer fusicoccin A

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein sigma Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P31947 (Residues: 1-231; Coverage: 93%)
Gene names: HME1, SFN
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
Potassium channel subfamily K member 9 Chain: P
Molecule details ›
Chain: P
Length: 6 amino acids
Theoretical weight: 857 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9NPC2 (Residues: 369-374; Coverage: 2%)
Gene names: KCNK9, TASK3

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: C2221
Unit cell:
a: 82.21Å b: 111.18Å c: 62.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.148 0.145 0.201
Expression systems:
  • Escherichia coli
  • Not provided